Microliciadeflexa and M.johnwurdackiana (Melastomataceae), two new species from the Brazilian Cerrado.

Microliciadeflexa sp. nov. and M.johnwurdackiana sp. nov. are described, illustrated and an updated identification key for the species of Microlicia from Clube Caça e Pesca Itororó de Uberlândia is provided. Microliciadeflexa occurs in Minas Gerais and Goiás States and is characterised by its linear-lanceolate and deflexed sepal on flower and immature fruit, long pedicel and indumentum of glandular trichomes, mixed with spherical, golden glands. Microliciajohnwurdackiana is endemic to Uberlândia and characterised by having indumentum of setose trichomes and spherical, golden glands, magenta petal with greenish abaxial surface at the apex and dimorphic stamens with bicolorous and tetrasporangiate anthers.

AbstractMicroliciadeflexa sp. nov. e M.johnwurdackiana sp. nov. são descritas, ilustradas e uma chave de identificação atualizada para as espécies de Microlicia do Clube Caça e Pesca Itororó de Uberlândia é fornecida. Microliciadeflexa ocorre nos estados de Minas Gerais e Goiás, e é caracterizada pela sépala linear-lanceolada e deflexa na flor e no fruto imaturo, pedicelo longo e indumento com tricomas glandulares misturados com glândulas esféricas, douradas. Microliciajohnwurdackiana é endêmica de Uberlândia, e caracterizada pelo indumento de tricomas setosos e glândulas esféricas, douradas, pétala magenta com a face abaxial esverdeada apenas no ápice e estames dimórficos com anteras bicolores e tetraesporangiadas.

Revisão de literatura das propriedades físico-químicas e biológicas de um cimento à base de silicato de cálcio / Literature review of physicochemical and biological properties of a calcium silicate base

Objetivo: O objetivo do presente trabalho foi revisar as publicações prévias sobre o EndoSequence BC Sealer com o intuito de avaliar os resultados obtidos por este cimento em relação às suas propriedades biológicas e físico-químicas, analisando-se, assim, de forma mais consistente, diversos âmbitos de sua atuação e possíveis desfechos de seu uso clínico realizar uma revisão de literatura sobre este material. Material e Métodos: Uma busca extensiva na literatura foi realizada para identificação de todos os artigos publicados sobre o cimento EndoSequence BC Sealer, considerando-se estudos que avaliaram suas propriedades biológicas e físico-químicas. A estratégia de busca foi fundamentada no acesso às bases de dados PubMed e Bireme. Resultados: Trinta e dois artigos satisfizeram os critérios de inclusão e foram utilizados na presente revisão. Os artigos demonstraram que o cimento possui radiopacidade, escoamento, solubilidade e alteração dimensional adequadas. Além disso, o cimento possui atividade antimicrobiana e é biocompatível. Conclusão: A avaliação dos trabalhos prévios do EndoSequence BC Sealer permite concluir que o cimento EndoSequence BC Sealer demonstrou performance satisfatória quanto as propriedades necessárias para um material obturador endodôntico.

Objective: The aim of this study was to review the previous publications of the EndoSequence BC Sealer in order to evaluate the results obtained using this sealer with regards to its biological and physicochemical properties; therefore, analyzing several areas of its performance and possible outcomes of its clinical use in a more reliable way. Material and Methods: A search of the literature was performed for the identification of all published articles using the EndoSequence BC Sealer, considering studies that evaluated its biological and physicochemical properties. The strategy of the search was based on access to the PubMed and Bireme databases. Results: Thirty-two manuscripts met the inclusion criteria and were used in this review. These manuscripts demonstrated that the sealer has adequate radiopacity, flow, solubility, and dimensional changes. Furthermore, the sealer has antimicrobial activity and is biocompatible. Conclusion: A review of the relevant literature suggests that the EndoSequence BC Sealer has demonstrated satisfactory performance in some important properties for a root canal sealer

Cytotoxicity and gelatinolytic activity of a new silicon-based endodontic sealer.

PURPOSE: To compare the cytotoxicity, gelatinolytic activity, and protein levels (MMP-2 and MMP-9) produced by 3T3 fibroblasts cells after stimulation with GuttaFlow 2 and AH Plus. METHODS: 3T3 fibroblasts were incubated with elutes of GuttaFlow 2 and AH Plus for 24 h. The cytotoxicity of tested materials was determined using the MTT and the LDH assay. Supernatants of cell cultures incubated with sealers were collected to determine the levels of MMP-2 and MMP-9 gelatinolytic activity by gelatin zymography. Cell lysates were used to determine MMP-2 and MMP-9 protein levels by Western Blot. Data were analyzed using ANOVA and Tukey test (P<0.05). RESULTS: AH Plus showed significantly less cell viability (mitochondrial activity of cells) than GuttaFlow 2 (P<0.01). Moreover, GuttaFlow 2 was noncytotoxic, showing no statistically significant difference in LDH leakage levels compared to the control group (P>0.05). Specific characterization of MMPs demonstrated that GuttaFlow 2 seemed not to affect MMP-2 levels compared with the control group, while AH Plus had elevated gelatinolytic activity and protein levels of MMP-2 as confirmed by quantitative measurements. No detectable gelatinolytic activity or protein levels of MMP-9 (92 kDa) was observed in any tested group. CONCLUSIONS: GuttaFlow 2 did not showed cytotoxic effects and did not induce MMP-2 or MMP-9 expression.